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Thermus phage Lalka27a

Хозяин
Thermus thermophilus HB27c
Морфотип
myovirus
Жизненный цикл
lysogenic
Тип генома
dsDNA
Длина генома, п.н.
71 251
GC-состав, %
70.6
Место выделения
Valentina hot springs, Kunashir Island
Accession
PQ109076

Карта генома

Lalka27a_refined71 251 п.н. · 84 открытые рамки считывания

CRISPR-кассета — 53 525..53 614 · 90 п.н. · консенсус повтора: GTTYYYCCCGTGCGCGCATGGAGGCACCGОриджин репликации — 63 072..63 307 · 236 п.н. · консенсус итерона: GGTGCRYBWBDTGYWYBRBHYUlx-like anti-restriction protein — 27..404 (−) · 378 п.н.DarB-like anti-restriction protein — 397..6 621 (−) · 6 225 п.н.ssDNA binding protein — 6 747..7 469 (+) · 723 п.н.virionic protein — 7 659..8 951 (−) · 1 293 п.н.hypothetical protein — 9 045..9 293 (+) · 249 п.н.hypothetical protein — 9 299..9 967 (+) · 669 п.н.hypothetical protein — 10 005..10 778 (−) · 774 п.н.hypothetical protein — 10 789..11 217 (−) · 429 п.н.hypothetical protein — 11 214..11 843 (−) · 630 п.н.portal protein — 11 895..13 658 (+) · 1 764 п.н.minor head protein — 13 645..14 595 (+) · 951 п.н.vWA domain-containing protein — 14 647..15 639 (+) · 993 п.н.SpoVR-like protein — 15 636..16 982 (+) · 1 347 п.н.serine protein kinase — 16 979..18 877 (+) · 1 899 п.н.hypothetical protein — 18 880..19 038 (+) · 159 п.н.hypothetical protein — 19 071..19 295 (+) · 225 п.н.TerL — 19 360..21 381 (+) · 2 022 п.н.hypothetical protein — 21 378..21 986 (−) · 609 п.н.hypothetical protein — 21 983..22 372 (−) · 390 п.н.dNMP kinase — 22 314..23 120 (−) · 807 п.н.nucleotidase — 23 113..23 745 (−) · 633 п.н.exonuclease — 23 732..25 003 (−) · 1 272 п.н.hypothetical protein — 25 143..25 457 (−) · 315 п.н.hypothetical protein — 25 457..25 876 (−) · 420 п.н.hypothetical protein — 25 939..26 199 (−) · 261 п.н.hypothetical protein — 26 220..26 435 (−) · 216 п.н.M23 family peptidase — 26 438..27 091 (−) · 654 п.н.hypothetical protein — 27 101..27 550 (−) · 450 п.н.hypothetical protein — 27 844..28 260 (−) · 417 п.н.hypothetical protein — 28 269..29 333 (−) · 1 065 п.н.hypothetical protein — 29 335..29 721 (−) · 387 п.н.hypothetical protein — 29 752..31 038 (−) · 1 287 п.н.baseplate wedge subunit — 31 039..32 100 (−) · 1 062 п.н.baseplate protein — 32 097..32 456 (−) · 360 п.н.hypothetical protein — 32 460..33 029 (−) · 570 п.н.hypothetical protein — 33 022..33 648 (−) · 627 п.н.PAAR-domain protein — 33 674..33 937 (−) · 264 п.н.putative zinc metallopeptidase — 33 937..34 371 (−) · 435 п.н.baseplate protein — 34 402..35 178 (−) · 777 п.н.hypothetical protein — 35 175..35 387 (−) · 213 п.н.putative helicase — 35 377..40 524 (−) · 5 148 п.н.tail protein — 40 588..41 970 (−) · 1 383 п.н.M23 family peptidase — 41 971..44 640 (−) · 2 670 п.н.hypothetical protein — 44 625..44 879 (−) · 255 п.н.tail assembly chaperone — 44 845..45 261 (−) · 417 п.н.virion structural protein — 45 332..45 793 (−) · 462 п.н.tail sheath — 45 807..47 546 (−) · 1 740 п.н.hypothetical protein — 47 560..47 769 (−) · 210 п.н.structural protein — 47 807..48 679 (−) · 873 п.н.hypothetical protein — 48 689..49 573 (−) · 885 п.н.hypothetical protein — 49 570..50 475 (−) · 906 п.н.tail fiber protein — 50 476..51 279 (−) · 804 п.н.yqbF-like protein — 51 309..51 713 (−) · 405 п.н.Cas12r1 — 51 815..53 509 (+) · 1 695 п.н.hypothetical protein — 53 619..53 750 (+) · 132 п.н.hypothetical protein — 53 741..53 932 (+) · 192 п.н.MCP — 53 960..55 468 (−) · 1 509 п.н.hypothetical protein — 55 465..56 112 (−) · 648 п.н.phage prohead protease — 56 126..57 046 (−) · 921 п.н.recombinase — 57 085..57 999 (+) · 915 п.н.phiFa_41 homolog — 58 009..58 227 (+) · 219 п.н.DNMTa — 58 174..59 019 (−) · 846 п.н.DNMT — 59 016..59 861 (−) · 846 п.н.hypothetical protein — 59 848..60 042 (−) · 195 п.н.hypothetical protein — 60 008..60 277 (−) · 270 п.н.primase — 60 274..60 897 (−) · 624 п.н.DnaB-like replicative helicase — 60 885..62 183 (−) · 1 299 п.н.replication initiation O-like protein — 62 173..63 108 (−) · 936 п.н.hypothetical protein — 63 195..63 326 (−) · 132 п.н.hypothetical protein — 63 443..63 535 (+) · 93 п.н.DNA polymerase processivity factor — 63 641..64 696 (−) · 1 056 п.н.hypothetical protein — 64 771..65 025 (−) · 255 п.н.RecT-like ssDNA annealing protein — 65 037..65 819 (−) · 783 п.н.hypothetical protein — 65 845..66 024 (−) · 180 п.н.hypothetical protein — 66 021..66 344 (−) · 324 п.н.hypothetical protein — 66 365..66 547 (−) · 183 п.н.hypothetical protein — 66 544..66 825 (−) · 282 п.н.hypothetical protein — 66 822..66 977 (−) · 156 п.н.hypothetical protein — 66 970..67 593 (−) · 624 п.н.hypothetical protein — 67 586..68 011 (−) · 426 п.н.transcriptional regulator — 68 033..68 437 (−) · 405 п.н.LexA-like regulator — 68 552..69 232 (+) · 681 п.н.putative Sie protein — 69 234..69 887 (+) · 654 п.н.integrase — 69 887..71 077 (+) · 1 191 п.н.

Последовательности

ФайлВидВерсияРазмер
Lalka27a_genome.gb.gzаннотация48 KB

Описание

Lalka27a, along with the related Lalka phages, encodes its own Type V CRISPR-Cas system, consisting of a Cas12r1 protein and a CRISPR array. It is worth to mention that the Lalka phages are among the few cultivated phage isolates known to harboring a CRISPR-Cas system (the only other examples are the Vibrio cholerae phages ICP1; all other cases reported to date are viral sequences recovered from metagenomes). The functions of the Lalka-encoded CRISPR-Cas system is still unclear; look at this note for the details.

With the exception of Lalka27b, which lacks an integrase gene, all Lalka phages appear to be capable of integrating into the host genome at the 3'-proximal segment of an alanine tRNA gene. In this aspect Lalka phages resemble other lysogenic Thermus phages, like phiMa, Cumys, Riverbug and Tsatsa.

Lalka27a infects T. thermophilus HB27c, yielding titers of up to 10¹⁰ PFU/ml upon lysis of a liquid culture. The closely related strain T. thermophilus HB27, however, appears to be partially resistant: it gives a reduced efficiency of plating and smaller plaques.

Lalka phages encode several virionic proteins which apparently are injected into the host cell upon the infection:

- Ulx (Lalka27a_1) - a homolog of phage P1 Ulx protein, which is required for protection against host type I restriction-modification (R-M) systems

- DarB (Lalka27a_2) - a homolog of P1 DarB, which is likewise required for protection against host type I R-M systems. The presence of DarB in Lalka27a virions was confirmed by proteomic analysis;

- Gp4 (Lalka27a_4) - detected in virions by proteomic analysis, but its functions are obscure. Given its proximity to the darB-ulx operon it might be possible that Gp4 is also involved in the protection against host type I RM systems;

- Lvh (large virion helicase Lalka27a_41) - a large protein with a predicted helicase domain. Homologs of Lalka Lvh are encoded by phiMa/Cumys phages, distant relatives of Lalka phages.

Примечания

Other closely related Lalka phages:

Lalka8

Lalka27b

Lalka27c

Lalka27d

Связанные публикации

  1. A Unique Type V CRISPR-Cas System Encoded by a Group of Thermus Viruses

    Anna B. Trofimova et al.

    bioRxiv (preprint)2026

  2. New Viruses Infecting Hyperthermophilic Bacterium Thermus thermophilus

    Matvey Kolesnik et al.

    Viruses2024

Проекты