LOCUS       phiKo                  11129 bp    DNA     linear   UNA 28-SEP-2026
DEFINITION  Thermus phage phiKo, complete genome.
ACCESSION   MH673671
KEYWORDS    .
SOURCE      Thermus phage phiKo
  ORGANISM  Thermus phage phiKo.
FEATURES             Location/Qualifiers
     source          1..11129
                     /country="Italy"
                     /db_xref=2301535
                     /host="Thermus thermophilus HB27"
                     /isolation_source="hot spring water"
                     /mol_type="genomic DNA"
                     /organism="Thermus phage phiKo"
                     /label="source"
     ITR             1..16
                     /note="<html><body>inverted terminal repeat</body></html>"
                     /label="ITR"
     CDS             85..618
                     /codon_start=1
                     /locus_tag="phiKo_26"
                     /product="hypothetical protein"
                     /protein_id="AYJ74701.1"
                     /transl_table=11
                     /translation="MTALMGTRRHAYEVLRKWATDEAGDSLAEAVAAVLPWWYLDRYL
                     DRLATDDGFREALGLAALELPGLGEVGRTEAYAAAKAILRALKDCPEDVLAWILLFGG
                     KYLSARGDRVEVGSKIHPGFEVSLSLVGGEIATVYVVVGGALGLESGEVGGKVAHLIG
                     AAARTVLAIALEGGREE*"
                     /label="phiKo_26"
     gene            85..618
                     /locus_tag="phiKo_26"
                     /label="phiKo_26"
     misc_feature    116..121
                     /Recognition_pattern="C^GTACG"
                     /label="BsiWI, Pfl23II, PspLI"
                     /note="Geneious type: restriction site"
     misc_feature    271..276
                     /Recognition_pattern="GAG^CTC"
                     /label="Ecl136II, Eco53kI, EcoICRI"
                     /note="Geneious type: restriction site"
     misc_feature    271..276
                     /Recognition_pattern="GAGCT^C"
                     /label="SacI, Psp124BI, SstI"
                     /note="Geneious type: restriction site"
     primer_bind     451..471
                     /Tm=59
                     /Description="<html><body></body></html>"
                     /label="phiKo_ORF26_qPCR_fwd"
     misc_feature    480..483
                     /Recognition_pattern="^AATT"
                     /label="MluCI, Sse9I, TasI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(553..570)
                     /Tm=59
                     /Description="<html><body></body></html>"
                     /label="phiKo_ORF26_qPCR_rev"
                     /note="Geneious type: primer_bind_reverse"
     CDS             615..902
                     /codon_start=1
                     /locus_tag="phiKo_25"
                     /product="hypothetical protein"
                     /protein_id="AYJ74700.1"
                     /transl_table=11
                     /translation="MTYPTIYHAMRHYYLPHEVAALLRGKAEVREEEGRPVVVDSEGR
                     VRLKAVSVLVEIQSRWGGATGLATPHWAFPMEDVEALLRALRRPAKGRKRG*"
                     /label="phiKo_25"
     gene            615..902
                     /locus_tag="phiKo_25"
                     /label="phiKo_25"
     misc_feature    663..671
                     /Recognition_pattern="CACNNN^GTG"
                     /label="DraIII, AdeI"
                     /note="Geneious type: restriction site"
     misc_feature    663..668
                     /Recognition_pattern="CACGAG(-5/-1)"
                     /label="BauI, BssSI, Bst2BI"
                     /note="Geneious type: restriction site"
     misc_feature    778..783
                     /Recognition_pattern="R^GATCY"
                     /label="BstX2I, BstYI, MflI, PsuI"
                     /note="Geneious type: restriction site"
     CDS             908..1102
                     /codon_start=1
                     /locus_tag="phiKo_24"
                     /product="hypothetical protein"
                     /protein_id="AYJ74699.1"
                     /transl_table=11
                     /translation="MILEVRRIGNGRWEVAVIRPGANPTYLVVYSYQDARELAALLGE
                     LIGEPVSVRQVREEVVGEDE*"
                     /label="phiKo_24"
     gene            908..1102
                     /locus_tag="phiKo_24"
                     /label="phiKo_24"
     CDS             1113..1496
                     /codon_start=1
                     /locus_tag="phiKo_23"
                     /product="hypothetical protein"
                     /protein_id="AYJ74698.1"
                     /transl_table=11
                     /translation="MGQELGAVELGSGKRYLTWKEYLEKVGDPKGYTGTHAVIRIWPP
                     AKYPSATLVFWVDDPNHPEGGYGVRKSIPPLLWSTLERAGFVPGKARKGGTLYLDFQG
                     GTMKLILEEDSGVYAPDKSGGWELV*"
                     /label="phiKo_23"
     gene            1113..1496
                     /locus_tag="phiKo_23"
                     /label="phiKo_23"
     misc_feature    1192..1198
                     /Recognition_pattern="GG^GWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     misc_feature    1520..1525
                     /Recognition_pattern="GTT^AAC"
                     /label="HpaI, KspAI"
                     /note="Geneious type: restriction site"
     CDS             1557..1775
                     /codon_start=1
                     /locus_tag="phiKo_22"
                     /product="hypothetical protein"
                     /protein_id="AYJ74697.1"
                     /transl_table=11
                     /translation="MPEEMGDGFILVYGRGETEEARENGYLPDVDEYRATFATFHEAY
                     MYAETIPVPTTIVYDEETDTWEVYVDYE*"
                     /label="phiKo_22"
     gene            1557..1775
                     /locus_tag="phiKo_22"
                     /label="phiKo_22"
     misc_feature    1666..1671
                     /Recognition_pattern="TCG^CGA"
                     /label="NruI, Bsp68I, BtuMI, RruI"
                     /note="Geneious type: restriction site"
     misc_feature    1758..1763
                     /Recognition_pattern="TAC^GTA"
                     /label="SnaBI, BstSNI, Eco105I"
                     /note="Geneious type: restriction site"
     CDS             1784..2731
                     /codon_start=1
                     /locus_tag="phiKo_21"
                     /product="DNA polymerase B"
                     /protein_id="AYJ74696.1"
                     /transl_table=11
                     /translation="MSALVVEPVWDWWEKAHHYPVEDDMHRQMAEDIFLNGAPEGAQM
                     AHVSPNLITIHYGGDRVRWERWRRLDAAGKLPSDYARAALKGAYRVWRPKWGRPEMEA
                     MRRWGYPPIHAQPGMYEDMAYVDVRSAYGTLYTLYWGVEYWPMRWLSLPRRAIVWPEE
                     LRANKLARNALYGLIRSEGLWAYRPGRKRLWLPNRMAYPQVALAVVDVLHTVAWRAVR
                     DMGAVYVMIDGIILPERNVPLLMDYVASMGLEGRVDAVGPANVEGVGAYAMPGKSTRR
                     RGNGRPFSNLKGEALGEWLEAATRRFLACRRYILGGEAL*"
                     /label="DNAP"
     gene            1784..2731
                     /locus_tag="phiKo_21"
                     /label="phiKo_21"
     misc_feature    1919..1924
                     /Recognition_pattern="CAC^GTG"
                     /label="PmaCI, AcvI, BbrPI, Eco72I, PmlI, PspCI"
                     /note="Geneious type: restriction site"
     misc_feature    2158..2163
                     /Recognition_pattern="C^GTACG"
                     /label="BsiWI, Pfl23II, PspLI"
                     /note="Geneious type: restriction site"
     misc_feature    2286..2291
                     /Recognition_pattern="GCAATG(2/0)"
                     /label="BsrDI, Bse3DI, BseMI"
                     /note="Geneious type: restriction site"
     primer_bind     2489..2508
                     /Tm=57
                     /Description="<html><body></body></html>"
                     /label="phiKo_ps_F"
     CDS             2728..3243
                     /codon_start=1
                     /locus_tag="phiKo_20"
                     /product="lysozyme"
                     /protein_id="AYJ74695.1"
                     /transl_table=11
                     /translation="MNWIEFWRSKKPTWRHRPVDPAYIVLHHTAGPVDQAPQAIWDYH
                     VKVRGWPHGGYHFLVYHDGTVVKMLPLSAQPICVGEYNHLAICIALVGNFVGGYPPEW
                     NERAPGWKSLAWLVRELRKHDSGLRLRLVRHKDLRPTKCPGTVTWEEALVRGGVPQEQ
                     VETLKVAGVIA*"
                     /modified_by="matve"
                     /label="endolysin"
     gene            2728..3243
                     /locus_tag="phiKo_20"
                     /label="phiKo_20"
     misc_feature    complement(2796..2815)
                     /Tm=59
                     /Description="<html><body></body></html>"
                     /label="phiKo_ps_R"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    2857..2862
                     /Recognition_pattern="CAC^GTG"
                     /label="PmaCI, AcvI, BbrPI, Eco72I, PmlI, PspCI"
                     /note="Geneious type: restriction site"
     primer_bind     2935..2944
                     /Tm=39
                     /Description="<html><body></body></html>"
                     /label="phiKo_M2.4_qPCR_fwd"
     misc_feature    2952..2957
                     /Recognition_pattern="CA^TATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     primer_bind     2999..3008
                     /Tm=36
                     /Description="<html><body></body></html>"
                     /label="phiKo_ORF03_qPCR_rev"
     misc_feature    3079..3084
                     /Recognition_pattern="GAG^CTC"
                     /label="Ecl136II, Eco53kI, EcoICRI"
                     /note="Geneious type: restriction site"
     misc_feature    3079..3084
                     /Recognition_pattern="GAGCT^C"
                     /label="SacI, Psp124BI, SstI"
                     /note="Geneious type: restriction site"
     misc_feature    3172..3184
                     /Recognition_pattern="(7/12)GAAGNNNNNNTAC(12/7)"
                     /label="BarI"
                     /note="Geneious type: restriction site"
     misc_feature    3191..3197
                     /Recognition_pattern="GG^GWCCC"
                     /label="KflI"
                     /note="Geneious type: restriction site"
     CDS             3259..3507
                     /codon_start=1
                     /locus_tag="phiKo_19"
                     /product="head morphogenesis protein"
                     /protein_id="AYJ74694.1"
                     /transl_table=11
                     /translation="MPRELEEKAAKLREQLNRLYERSDALMAEIKRLQDEAAGKQGME
                     RWTILRRAYKLKDEWNSVAKQIMNMGVRLARIEEALGR*"
                     /modified_by="matve"
                     /label="HMP"
     gene            3259..3507
                     /locus_tag="phiKo_19"
                     /label="phiKo_19"
     misc_feature    3306..3318
                     /Recognition_pattern="(7/12)GAACNNNNNNTAC(12/7)"
                     /label="PsrI"
                     /note="Geneious type: restriction site"
     primer_bind     3333..3342
                     /Tm=20
                     /Description="<html><body></body></html>"
                     /label="IC_spacer_2.4"
     CDS             3510..3731
                     /codon_start=1
                     /locus_tag="phiKo_18"
                     /product="hypothetical protein"
                     /protein_id="AYJ74693.1"
                     /transl_table=11
                     /translation="MEELCLIAGPVASLVVQALKRFPVVQRYPKVVLAALTGAYVLVQ
                     GTGWECWVAAFASGIAGYEVVIKPVAKKE*"
                     /label="phiKo_18"
     gene            3510..3731
                     /locus_tag="phiKo_18"
                     /label="phiKo_18"
     misc_feature    3533..3539
                     /Recognition_pattern="CG^GWCCG"
                     /label="RsrII, CpoI, CspI, Rsr2I"
                     /note="Geneious type: restriction site"
     misc_feature    3669..3674
                     /Recognition_pattern="GAATGC(1/-1)"
                     /label="BsmI, Mva1269I, PctI"
                     /note="Geneious type: restriction site"
     misc_feature    3733..3738
                     /Recognition_pattern="GCAATG(2/0)"
                     /label="BsrDI, Bse3DI, BseMI"
                     /note="Geneious type: restriction site"
     CDS             3736..3921
                     /codon_start=1
                     /locus_tag="phiKo_17"
                     /product="ribonuclease Z"
                     /protein_id="AYJ74692.1"
                     /transl_table=11
                     /translation="MDWVQAIRDIGFPIVVSLLLLLKLDASLNRLREAVEENTRILRV
                     GFRVMNGHKEEDVHGKR*"
                     /modified_by="matve"
                     /label="RNase Z"
     gene            3736..3921
                     /locus_tag="phiKo_17"
                     /label="phiKo_17"
     misc_feature    3799..3804
                     /Recognition_pattern="C^TTAAG"
                     /label="AflII, BfrI, BspTI, BstAFI, MspCI, Vha464I"
                     /note="Geneious type: restriction site"
     misc_feature    3802..3807
                     /Recognition_pattern="A^AGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     CDS             3925..4770
                     /codon_start=1
                     /locus_tag="phiKo_14_17"
                     /product="hypothetical protein"
                     /transl_table=11
                     /translation="MGSPEADKERLGSLTAEPGGEGGGGPRGGAGSGGGGGGSRGGGG
                     GSRGGSGGGGRSARRGRGGSGSGSGGRSGGGGEGGLGPAVPDSGGSASAGARELGEDA
                     AAEALPSPTPRPVAPPRASDAAALGALGEMLLKAIMGGLSLVLGPHWAVSDEEAKAWG
                     KALEDWAKAQPPKRRKALSKALGAVPTVNLIGTTALIVAPRVQATLQMRAAKKEAEDG
                     RKGRAGSGVPGTLGADSPGSPEKPYGYDFAGAVKRLSEQDAGGAGSGDSGGNGGVRGA
                     PKARA*"
                     /label="phiKo_14_17"
     gene            3925..4770
                     /locus_tag="phiko_14_17"
                     /label="phiko_14_17"
     misc_feature    3971..3979
                     /Recognition_pattern="CAGNNN^CTG"
                     /label="AlwNI, CaiI, PstNI"
                     /note="Geneious type: restriction site"
     misc_feature    4166..4176
                     /Recognition_pattern="GACNNN^NNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    4183..4188
                     /Recognition_pattern="T^CCGGA"
                     /label="AccIII, Aor13HI, BseAI, Bsp13I, BspEI, Kpn2I,
                     MroI"
                     /note="Geneious type: restriction site"
     misc_feature    complement(4397..4434)
                     /Tm=74
                     /Description="<html><body></body></html>"
                     /label="Ko_M16.2"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(4530..4547)
                     /Tm=60
                     /Description="<html><body></body></html>"
                     /label="phiKo_M2.4_qPCR_rev"
                     /note="Geneious type: primer_bind_reverse"
     CDS             4776..5387
                     /codon_start=1
                     /locus_tag="phiKo_14"
                     /product="DNA packaging ATPase"
                     /protein_id="AYJ74689.1"
                     /transl_table=11
                     /translation="MDWRRIGHSDRVAVVGTTGAGKSTLLRAMMASRPWVLLYDPKGE
                     WEGEEWLTVREAAKLPRKPHPRVRYFPLPDELRDKEALDDFFWWAYYAKNLTVVVDEA
                     YLATLAGAWLPPGYQACLAQGRSRGVAVWTATQRPYKIPQTILSEAEHWFVFRLNAPQ
                     DRWAVHQWTGLDSRLISPENLPKRVWYYVSEGQVYGPYMLRLE*"
                     /modified_by="matve"
                     /label="DNA packaging ATPase"
     gene            4776..5387
                     /locus_tag="phiKo_14"
                     /label="phiKo_14"
     misc_feature    5268..5273
                     /Recognition_pattern="G^TGCAC"
                     /label="ApaLI, Alw44I, VneI"
                     /note="Geneious type: restriction site"
     CDS             5401..6426
                     /codon_start=1
                     /locus_tag="phiKo_13"
                     /product="putative major capsid protein"
                     /protein_id="AYJ74688.1"
                     /transl_table=11
                     /translation="MAGNAQQASGGGTIPFRYGSRMRIQRVGFIPFQENTQAPPLELP
                     RVGFLAAVVLEWRGAISGTGVSVGPRGPWALVRRLRLGLNLGAVNIYDTSGYGNYLIN
                     LLNGRDFTKHPLYDSTSRWHYHIPVAVNLGENATTGLVLLQDPEVRATVEVTWGALAD
                     AFNGTGLSVDSGAGLDVYYVYFEVPDLRKVSAPPLVLHRVLEDTQPITQTGDNAYLVP
                     RQGILLQLAHTLTLNGAFSNAWDRAFVRLNRTDTIMEVIPGVHYALDLQGRNAQTSPI
                     TPTHVIFHDLFRAMGALDRGDLRDALDTEAVTTTESVVTVSQSATLGANNNFLGAIRR
                     IIQPIRV*"
                     /modified_by="matve"
                     /label="MCP"
     gene            5401..6426
                     /locus_tag="phiKo_13"
                     /label="phiKo_13"
     misc_feature    5463..5472
                     /Recognition_pattern="GATNN^NNATC"
                     /label="BsaBI, Bse8I, BseJI"
                     /note="Geneious type: restriction site"
     misc_feature    5605..5612
                     /Recognition_pattern="CC^TCGAGG"
                     /label="AbsI"
                     /note="Geneious type: restriction site"
     misc_feature    5605..5612
                     /Recognition_pattern="VC^TCGAGB"
                     /label="PspXI"
                     /note="Geneious type: restriction site"
     misc_feature    5606..5611
                     /Recognition_pattern="C^TCGAG"
                     /label="XhoI, PaeR7I, Sfr274I, SlaI"
                     /note="Geneious type: restriction site"
     misc_feature    5607..5610
                     /Recognition_pattern="T^CGA"
                     /label="TaqI"
                     /note="Geneious type: restriction site"
     misc_feature    5627..5632
                     /Recognition_pattern="TGC^GCA"
                     /label="Acc16I, FspI, NsbI"
                     /note="Geneious type: restriction site"
     misc_feature    5746..5756
                     /Recognition_pattern="GACNNN^NNGTC"
                     /label="Eam1105I, AhdI, BmeRI, DriI"
                     /note="Geneious type: restriction site"
     misc_feature    6371..6384
                     /Recognition_pattern="(7/12)GAANNNNNNNTTGG(11/6)"
                     /label="AjuI"
                     /note="Geneious type: restriction site"
     CDS             6440..6814
                     /codon_start=1
                     /locus_tag="phiKo_12"
                     /product="hypothetical protein"
                     /protein_id="AYJ74687.1"
                     /transl_table=11
                     /translation="MLSDYVALNNVLPSVGGWSLGEQATAGLQDATQPATTASGKTPT
                     GQGALLEGQSPLVTFLVVLGFLLLLMWLAHTYGEPGEYSNLKASAYNVLFIGLTAALF
                     IPLLKVAAVKIPGPWTSYFLSI*"
                     /label="phiKo_12"
     gene            6440..6814
                     /locus_tag="phiKo_12"
                     /label="phiKo_12"
     misc_feature    6467..6472
                     /Recognition_pattern="AA^CGTT"
                     /label="AclI, Psp1406I"
                     /note="Geneious type: restriction site"
     CDS             6823..6996
                     /codon_start=1
                     /locus_tag="phiKo_11"
                     /product="hypothetical protein"
                     /protein_id="AYJ74686.1"
                     /transl_table=11
                     /translation="MWEYLQAKAQEWLGRTGLTFQVVLVLVLLYLGYRAGTPWLIAAI
                     LVSMVVLAGGGGE*"
                     /label="phiKo_11"
     gene            6823..6996
                     /locus_tag="phiKo_11"
                     /label="phiKo_11"
     misc_feature    6834..6841
                     /Recognition_pattern="CCTGCA^GG"
                     /label="Sse8387I, SbfI, SdaI"
                     /note="Geneious type: restriction site"
     CDS             6983..7123
                     /codon_start=1
                     /locus_tag="phiKo_10"
                     /product="hypothetical protein"
                     /protein_id="AYJ74685.1"
                     /transl_table=11
                     /translation="VAVSRLGWAIIAAVVLALTATYFRPLWIVVGLVILIFLLNAVGG
                     GR*"
                     /label="phiKo_10"
     gene            6983..7123
                     /locus_tag="phiKo_10"
                     /label="phiKo_10"
     CDS             7125..7277
                     /codon_start=1
                     /locus_tag="phiKo_09"
                     /product="hypothetical protein"
                     /protein_id="AYJ74684.1"
                     /transl_table=11
                     /translation="MDTVEKIALTALAIVAVIVVLQRGTAAAQVVNAIGSTYTNLVKT
                     LVSAPK*"
                     /label="phiKo_09"
     gene            7125..7277
                     /locus_tag="phiKo_09"
                     /label="phiKo_09"
     CDS             7288..7602
                     /codon_start=1
                     /locus_tag="phiKo_08"
                     /product="hypothetical protein"
                     /protein_id="AYJ74683.1"
                     /transl_table=11
                     /translation="MCNREVALPRSRYAQALRDEAGLPTRPYDYYSRPSMYPGPVGPV
                     PAPPTIPVPTPIGILPGYRGELSALKYQGVVPREHPAGPYAPEGGYITWADVVKRRGD
                     GG*"
                     /label="phiKo_08"
     gene            7288..7602
                     /locus_tag="phiKo_08"
                     /label="phiKo_08"
     misc_feature    7359..7364
                     /Recognition_pattern="CACGAG(-5/-1)"
                     /label="BauI, BssSI, Bst2BI"
                     /note="Geneious type: restriction site"
     CDS             7592..8071
                     /codon_start=1
                     /locus_tag="phiKo_07"
                     /product="peptidase"
                     /protein_id="AYJ74682.1"
                     /transl_table=11
                     /translation="MEVKDYLPFAVVGGGALLAYLLLKPQPYTVVNPPTPQGPSEDAR
                     LNAVVALTQLAVERERARWEYAATVEAAKAQAEYELRARQIEAEQEARRIQAEKEVQQ
                     KAADAQFWGTFWSGLFGIIGSGLALFAGLSDEALYNRLPYPPYIGHGGQLAALSRRR*
                     "
                     /modified_by="matve"
                     /label="peptidase"
     gene            7592..8071
                     /locus_tag="phiKo_07"
                     /label="phiKo_07"
     misc_feature    7754..7759
                     /Recognition_pattern="GCAGTG(2/0)"
                     /label="BtsI"
                     /note="Geneious type: restriction site"
     misc_feature    7924..7929
                     /Recognition_pattern="AA^CGTT"
                     /label="AclI, Psp1406I"
                     /note="Geneious type: restriction site"
     CDS             8074..8547
                     /codon_start=1
                     /locus_tag="phiKo_06_7"
                     /product="hypothetical protein"
                     /transl_table=11
                     /translation="MGIAIEPIDSGPCVDSDCYYARGERLTWWEAPPPPPPSNPALDP
                     PPPPPPQREERPRIDPPPTLFDQPSYSAEPPPSPPPSTPSPPVDPEPPPDPLPPVTSD
                     DPLYSVIGELLGGREPTAQPEPIVLAPPPSNPSLALWIMALAVVGVLGYAWLQRR*"
                     /label="phiKo_06_7"
     gene            8074..8547
                     /locus_tag="phiKo_06_7"
                     /label="phiKo_06_7"
     misc_feature    8098..8107
                     /Recognition_pattern="GACNN^NNGTC"
                     /label="PshAI, BoxI, BstPAI"
                     /note="Geneious type: restriction site"
     misc_feature    8152..8158
                     /Recognition_pattern="A^CCWGGT"
                     /label="SexAI, CsiI, MabI"
                     /note="Geneious type: restriction site"
     misc_feature    8258..8269
                     /Recognition_pattern="(11/13)CAANNNNNGTGG(12/10)"
                     /label="CspCI"
                     /note="Geneious type: restriction site"
     CDS             8550..8801
                     /codon_start=1
                     /locus_tag="phiKo_06"
                     /product="hypothetical protein"
                     /protein_id="AYJ74681.1"
                     /transl_table=11
                     /translation="MDWGKLIGFRRYQRQNPDAYVAPTSPLPEWPPLPQTGTFAHWGD
                     DVLANLSGGAARAMWDWPARYATVEAFTPPEVVLRWPTP*"
                     /label="phiKo_06"
     gene            8550..8801
                     /locus_tag="phiKo_06"
                     /label="phiKo_06"
     misc_feature    8689..8694
                     /Recognition_pattern="TGG^CCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     CDS             8786..9343
                     /codon_start=1
                     /locus_tag="phiKo_05"
                     /product="hypothetical protein"
                     /protein_id="AYJ74680.1"
                     /transl_table=11
                     /translation="VAYALDVYQLLRDCTGRDDLARLGTAIFLHEKGPQGYEAGYGYT
                     DSGPLPEWRGFQKQIQGVCSELTRFFQGRPVNAYTVREFWREEWRASDIGWPQKVWAW
                     YLRLAGAPTDKLAQMPIGRGEASRPSPLLPRIPTVEDKLQGSLSPRVQEVLEQRTPEA
                     VRKALLIGLGAIGLAVVWYEVGRRV*"
                     /label="phiKo_05"
     gene            8786..9343
                     /locus_tag="phiKo_05"
                     /label="phiKo_05"
     misc_feature    9059..9064
                     /Recognition_pattern="GAT^ATC"
                     /label="EcoRV, Eco32I"
                     /note="Geneious type: restriction site"
     CDS             9340..9771
                     /codon_start=1
                     /locus_tag="phiKo_04"
                     /product="hypothetical protein"
                     /protein_id="AYJ74679.1"
                     /transl_table=11
                     /translation="MSLTPQGVSGNSVGGDSSSSAWPSTLVAPGTIYHLGRWTGQTLR
                     KLILSTATDSIVYGRVQIALDYPRGGLHPGNNALPNALVDLPLNFSPQHPIISLDFTP
                     GLVTVLPGESIYLIVYDFMSTTSAAITWGLNMFWEPLGGER*"
                     /label="phiKo_04"
     gene            9340..9771
                     /locus_tag="phiKo_04"
                     /label="phiKo_04"
     misc_feature    9389..9395
                     /Recognition_pattern="GCTCTTC(1/4)"
                     /label="SapI, BspQI, LguI, PciSI"
                     /note="Geneious type: restriction site"
     misc_feature    9627..9632
                     /Recognition_pattern="A^AGCTT"
                     /label="HindIII"
                     /note="Geneious type: restriction site"
     misc_feature    9648..9653
                     /Recognition_pattern="A^CTAGT"
                     /label="SpeI, AhlI, BcuI"
                     /note="Geneious type: restriction site"
     misc_feature    9698..9703
                     /Recognition_pattern="T^CATGA"
                     /label="BspHI, CciI, PagI"
                     /note="Geneious type: restriction site"
     misc_feature    9713..9718
                     /Recognition_pattern="CCGC^GG"
                     /label="SacII, Cfr42I, KspI, Sfr303I, SgrBI"
                     /note="Geneious type: restriction site"
     CDS             9768..10577
                     /codon_start=1
                     /locus_tag="phiKo_03"
                     /product="tail protein"
                     /protein_id="AYJ74678.1"
                     /transl_table=11
                     /translation="MSLEIFPTYPEIRTEQLSAVEWDKLPNLSTLVISVIRNAPTITP
                     SPGDWLLGVLAGGTEVGKLPIGQADGVPLLNASGALASADAYLSRGSVTSAVDWNTLT
                     NAGTYEIASGAFGTGSANTPPAVTQQGHLLVLKTSGATTQVYVPKSADPGIYWRQYAG
                     GTWSSWATAGVMYGSNSNGSYIRFADGTQICWREATWEMPTGWSTGPSVPWPAAFVAT
                     PRVWLNYTRSGWRIILSDITNNSTTIQLSAYNFDTTVTPYRVDILAIGRWK*"
                     /label="phiKo_03"
     gene            9768..10577
                     /locus_tag="phiKo_03"
                     /label="phiKo_03"
     primer_bind     9847..9866
                     /Tm=59
                     /Description="<html><body></body></html>"
                     /label="phiKo_ORF03_qPCR_fwd"
     misc_feature    9907..9917
                     /Recognition_pattern="(8/13)GAGNNNNNCTC(13/8)"
                     /label="BplI"
                     /note="Geneious type: restriction site"
     misc_feature    9936..9946
                     /Recognition_pattern="(10/15)ACNNNNGTAYC(12/7)"
                     /label="BaeI"
                     /note="Geneious type: restriction site"
     misc_feature    9971..9975
                     /Recognition_pattern="CGWCG^"
                     /label="Hpy99I"
                     /note="Geneious type: restriction site"
     misc_feature    10006..10011
                     /Recognition_pattern="TCG^CGA"
                     /label="NruI, Bsp68I, BtuMI, RruI"
                     /note="Geneious type: restriction site"
     misc_feature    10016..10020
                     /Recognition_pattern="CGWCG^"
                     /label="Hpy99I"
                     /note="Geneious type: restriction site"
     misc_feature    10084..10089
                     /Recognition_pattern="CA^TATG"
                     /label="NdeI, FauNDI"
                     /note="Geneious type: restriction site"
     misc_feature    10113..10118
                     /Recognition_pattern="A^CCGGT"
                     /label="AgeI, AsiGI, BshTI, CspAI, PinAI"
                     /note="Geneious type: restriction site"
     misc_feature    10155..10161
                     /Recognition_pattern="CACCTGC(4/8)"
                     /label="AarI, PaqCI"
                     /note="Geneious type: restriction site"
     misc_feature    10187..10196
                     /Recognition_pattern="CACNN^NNGTG"
                     /label="OliI, AleI"
                     /note="Geneious type: restriction site"
     misc_feature    10187..10196
                     /Recognition_pattern="CAYNN^NNRTG"
                     /label="MslI, RseI, SmiMI"
                     /note="Geneious type: restriction site"
     misc_feature    10251..10257
                     /Recognition_pattern="A^CCWGGT"
                     /label="SexAI, CsiI, MabI"
                     /note="Geneious type: restriction site"
     misc_feature    10270..10275
                     /Recognition_pattern="C^GGCCG"
                     /label="BseX3I, BstZI, EagI, EclXI, Eco52I"
                     /note="Geneious type: restriction site"
     misc_feature    10377..10382
                     /Recognition_pattern="W^GTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     misc_feature    10414..10419
                     /Recognition_pattern="TGG^CCA"
                     /label="BalI, MlsI, MluNI, Mox20I, MscI, Msp20I"
                     /note="Geneious type: restriction site"
     misc_feature    10488..10493
                     /Recognition_pattern="AGT^ACT"
                     /label="ScaI, BmcAI, ZrmI"
                     /note="Geneious type: restriction site"
     misc_feature    10488..10493
                     /Recognition_pattern="W^GTACW"
                     /label="TatI"
                     /note="Geneious type: restriction site"
     misc_feature    10500..10505
                     /Recognition_pattern="C^AATTG"
                     /label="MfeI, MunI"
                     /note="Geneious type: restriction site"
     misc_feature    10501..10504
                     /Recognition_pattern="^AATT"
                     /label="MluCI, Sse9I, TasI"
                     /note="Geneious type: restriction site"
     CDS             10574..10867
                     /codon_start=1
                     /locus_tag="phiKo_02"
                     /product="hypothetical protein"
                     /protein_id="AYJ74677.1"
                     /transl_table=11
                     /translation="VIRIHYVPQVGLPGKRLVYSWSGRVLTVRLEPDGVEDVYDLSVL
                     GPGDEVVGVETETLPFTPILSARLEIDGDLHVTLLHWYEGGEEPELAEEVLDG*"
                     /label="phiKo_02"
     gene            10574..10867
                     /locus_tag="phiKo_02"
                     /label="phiKo_02"
     misc_feature    10739..10744
                     /Recognition_pattern="GGTCTC(1/5)"
                     /label="Eco31I, BsaI, Bso31I, BspTNI"
                     /note="Geneious type: restriction site"
     CDS             10860..11111
                     /codon_start=1
                     /locus_tag="phiKo_01"
                     /product="hypothetical protein"
                     /protein_id="AYJ74676.1"
                     /transl_table=11
                     /translation="MARLKIKTHQDRIREAWAALRAERDRRLAETDWVVARAYERGEP
                     VPEAWAAYRQALRDLPALVGEEDLLAGRIPWPEPPGGAS*"
                     /label="phiKo_01"
     gene            10860..11111
                     /locus_tag="phiKo_01"
                     /label="phiKo_01"
     misc_feature    10965..10972
                     /Recognition_pattern="GCCC^GGGC"
                     /label="SrfI"
                     /note="Geneious type: restriction site"
     ITR             complement(11114..11129)
                     /note="<html><body>inverted terminal
                     repeat<br/></body></html>"
                     /label="ITR"
ORIGIN      
        1 cagggttggg gggagtatat actttgggca tgggggttgc gtatcgtatg cccgtgtgat
       61 attgtgttct cggaggtgag cgcgatgacg gctctcatgg gcacccgcag gcacgcgtac
      121 gaggtactga ggaagtgggc cacggatgag gccggggact ccttggccga ggcggtggcg
      181 gcggtactcc cctggtggta cttggaccgc tacctggacc gccttgctac tgatgacggc
      241 ttccgggagg ccttgggcct cgcggccctt gagctccccg gtctggggga ggttggccgt
      301 accgaggcct acgccgccgc caaagccatc cttagggcgc taaaagattg ccccgaagat
      361 gtcttggcgt ggatactact ctttggcgga aagtacctga gcgcgcgtgg agatcgcgtg
      421 gaagttggta gcaaaatcca ccctgggttt gaagtgtctc tgtccctggt gggcggggaa
      481 attgccacag tctatgtcgt ggttggcggg gcgctcgggc ttgagtcggg ggaagtgggg
      541 gggaaggtgg cccatcttat tggcgcggcg gctcgcacgg tgctcgccat cgccctggag
      601 ggagggaggg aggaatgacc taccccacca tctaccacgc tatgcgccac tactatctcc
      661 cccacgaggt ggccgccctc ctccggggca aggcggaggt gcgggaggag gaggggcggc
      721 ccgtagtggt ggactccgag gggagggtgc ggctcaaggc ggtgagcgtc ctggtggaga
      781 tccagagccg ttgggggggc gctactggcc tcgctacccc tcattgggct tttcccatgg
      841 aagacgtgga ggctctcttg cgggcgctta ggaggcctgc taagggaagg aaaagggggt
      901 gattaggatg attttggaag tgcggcgtat aggtaatggg cgctgggaag tggccgtcat
      961 tcgccccggg gcgaacccca cgtatctggt ggtctactcc taccaggacg ctcgggaact
     1021 cgcggcgctc ctcggggagc tgattgggga gcccgtgagc gtgcggcagg ttagggaaga
     1081 ggttgtagga gaagacgagt aggaggtgag ccatgggaca ggaactcggt gcggtggaac
     1141 tcggtagcgg caagaggtac ctgacgtgga aggagtacct ggagaaggtg ggggacccca
     1201 agggctacac gggcacccac gcggtcatcc gcatttggcc ccccgccaag tacccctcgg
     1261 ccaccctggt cttttgggtg gacgacccca atcaccctga agggggttac ggcgtgagga
     1321 agtccattcc ccctctgctt tggtccaccc tggagagggc ggggtttgtt ccggggaagg
     1381 ctcggaaggg cggcacgctc tacctggact tccagggggg caccatgaaa ctcatcctgg
     1441 aggaagatag cggggtttac gcccccgaca agtcgggagg ctgggagctg gtgtaagggg
     1501 taaactaggg gggagagggg ttaaccttct cccccttgcc tttgaggagg tgagtcatgc
     1561 ccgaggaaat gggagacggc ttcattttgg tctacggcag gggggaaact gaggaggccc
     1621 gggagaacgg ctatcttccc gatgtggacg agtaccgggc cacattcgcg actttccacg
     1681 aagcgtacat gtacgcagaa actatcccgg taccaacaac aatcgtctac gatgaggaaa
     1741 cggacacctg ggaagtctac gtagactacg agtaggagga gagatgagcg cccttgtggt
     1801 ggaacccgtg tgggactggt gggagaaagc ccaccactac ccggtagaag acgacatgca
     1861 caggcaaatg gctgaggaca ttttccttaa cggggccccc gagggcgctc agatggccca
     1921 cgtgagcccc aacctcatca caatccatta tgggggggac cgggtgcggt gggagcgctg
     1981 gcggcgcttg gacgcggcag ggaaactccc atccgattac gctcgggcgg ccctaaaggg
     2041 ggcataccga gtgtggaggc ctaaatgggg gcggcccgag atggaggcca tgcggcgatg
     2101 gggctacccc cccatacacg cccaaccggg catgtacgag gatatggcct atgtggacgt
     2161 acggagcgcc tacggcacgc tctacacgct ctattggggc gtggagtatt ggcccatgcg
     2221 gtggctctct cttccgaggc gggccatagt gtggcctgag gagttgcggg cgaataagtt
     2281 ggcccgcaat gcgctttatg gactgatacg ctctgagggg ctttgggcgt ataggcctgg
     2341 gcgaaaacgc ttatggttgc cgaacaggat ggcctacccc caagtggctc ttgccgtggt
     2401 ggacgtgttg cacacggtag cgtggagggc ggtgcgggac atgggggcgg tctacgtgat
     2461 gattgacggg attatcttgc cggagcgcaa cgtacccctc ctcatggact acgtggcttc
     2521 catggggctt gaggggcgcg tggacgcggt gggcccggcc aacgtggagg gcgtaggagc
     2581 ctacgctatg ccggggaaat ctacgaggag gcgcggtaac ggtcgccctt tttccaatct
     2641 gaagggggag gcccttgggg aatggcttga ggcggcaaca cggcggtttt tggcgtgtag
     2701 gcggtatata ctgggagggg aggcgctatg aactggattg agttttggcg gtctaagaag
     2761 cccacttgga ggcataggcc tgttgacccc gcctatattg tcctgcacca cacggcgggg
     2821 cccgtggacc aagctcctca ggctatttgg gactatcacg tgaaggtaag agggtggccc
     2881 cacgggggct accacttcct ggtctaccac gatgggaccg tggtgaagat gttgccccta
     2941 agcgcccaac ccatatgcgt aggggaatac aatcacctag ccatttgcat cgcgctggtg
     3001 ggcaactttg tgggcggcta ccctcctgag tggaacgagc gggcccccgg atggaagtct
     3061 ttggcgtggc ttgtgcggga gctcaggaag cacgacagcg gattgcggtt gaggttagtg
     3121 cggcacaaag accttaggcc aacgaagtgc cctggaacag ttacgtggga ggaagcccta
     3181 gtacgggggg gggtccccca agaacaggtg gagacgctga aagtggcggg cgtgatagca
     3241 taaggaaagg agggacgtat gccgcgtgaa ctggaggaaa aggcggccaa actgcgggaa
     3301 cagttgaaca ggctctacga gcgttccgat gcactcatgg ctgagattaa gcgcctacag
     3361 gatgaggcgg cagggaagca gggcatggag cggtggacca tcttgcggcg ggcgtacaag
     3421 ctcaaggacg agtggaacag cgttgcaaag cagattatga acatgggggt taggctagcc
     3481 cgcattgagg aggcgttggg gaggtgagca tggaggaact ttgccttatt gccggtccgg
     3541 ttgctagttt ggtcgtccag gccttgaaga ggttcccggt ggtacagcgt taccctaaag
     3601 tggtcctcgc cgctttaact ggggcttacg tcctggttca gggtacgggc tgggagtgct
     3661 gggtggcggc attcgcttcg ggcatagctg gatatgaagt tgtgattaag cccgtggcga
     3721 agaaagagta gggcaatgga ctgggtgcaa gccattaggg acataggctt ccccatcgtg
     3781 gtatctctac tcctcctgct taagcttgac gctagcctaa acaggcttag ggaggcagta
     3841 gaggagaaca cccgcatatt gcgggtaggg tttcgcgtga tgaacgggca caaggaggag
     3901 gatgtccatg gaaagaggtg aggtatggga agccctgaag cggataagga acgacttgga
     3961 agccttactg cagaacctgg aggagaaggc ggaggcggtc cccgaggagg cgcaggaagc
     4021 ggtggaggag gtggcggaag ccgtggagga ggtggcggaa gccgtggagg aagcggtggg
     4081 ggaggccgtt cagcccgtag aggaaggggc ggaagcggta gcggaagcgg tggaagaagc
     4141 ggtggtggag gtgagggagg ccttggaccc gccgtccccg actccggagg aagtgcctcc
     4201 gccggagccc gtgaacttgg agaagacgcg gcggcagaag ccctacctag cccgactcct
     4261 cggcctgtag ctcctccaag ggcttccgat gcggcggccc tcggagccct cggggaaatg
     4321 ttgctgaagg ccatcatggg gggcttgagt ctggtcctag gcccccattg ggcggtatcg
     4381 gacgaggagg ctaaagcgtg gggcaaggcc ctggaggatt gggctaaagc tcaaccccct
     4441 aagcggcgta aagccttgag taaagcgctt ggggcggtgc ctacggtgaa cctcatcggc
     4501 actacggccc tcatcgtggc ccctagggtg caagcgaccc tgcagatgcg ggccgcgaaa
     4561 aaggaggcgg aagatgggcg taaaggcaga gctggtagcg gtgttccagg aactcttggc
     4621 gcggatagcc ccggaagccc ggaaaaaccc tatggctatg attttgctgg agcagttaag
     4681 cggctatctg aacaggatgc cggaggcgca ggctcgggag atagcggcgg taatggtggg
     4741 gtacggggag cgcctaaggc gcgcgcttga tgctaatgga ctggaggcgg atagggcata
     4801 gcgatagggt tgccgtggtg ggcaccacgg gggcggggaa gtccactctc cttagggcga
     4861 tgatggcttc ccgcccttgg gtgctcctct acgatcctaa gggggaatgg gagggggaag
     4921 aatggctcac cgtgcgggag gccgctaaac tcccccgcaa gccccacccc cgggtgcgct
     4981 acttccccct ccccgatgag cttagggaca aagaagccct tgacgacttc ttttggtggg
     5041 cctactacgc taagaacctg acggtcgttg tggacgaggc ttatctagct actctcgcgg
     5101 gcgcgtggct tccacccggc taccaggcat gtctggcaca agggagaagc cgaggggtgg
     5161 ccgtgtggac ggccacgcaa cgcccctaca aaatcccgca gactatcctc tcggaggcag
     5221 agcattggtt tgtcttccgg ctcaatgccc cccaggacag atgggcggtg caccaatgga
     5281 cgggattgga ctctaggctt atcagcccgg aaaacctccc caagcgggtg tggtattatg
     5341 tgagtgaagg gcaagtctat ggcccttaca tgttgaggtt agaatgagga ggtagaggat
     5401 atggctggaa acgctcagca ggctagcggc ggcggcacca tccccttcag gtacggtagc
     5461 cggatgcgta tccagagggt gggcttcatc ccgttccagg agaacactca agcccctcct
     5521 ctggagctac cgcgtgtagg ctttttggcg gcggtggtgt tggagtggcg tggggcgatt
     5581 agtggaactg gtgtttccgt ggggcctcga ggcccgtggg cgctggtgcg caggctccgc
     5641 cttgggctca acctcggggc ggtcaacatc tacgacacgt ccgggtacgg caactacctt
     5701 atcaacctgc tcaatgggcg ggacttcact aagcaccccc tttatgactc cacgtcccgg
     5761 tggcactacc acatccccgt agcggtcaac ctcggggaga acgccactac cgggttagtg
     5821 ctcctccaag accccgaggt gcgggccacg gtggaagtta cgtggggggc cctcgctgac
     5881 gcttttaacg gcacggggct ttccgtggat tccggcgcgg gccttgacgt ttactatgtc
     5941 tactttgagg tgccggacct caggaaggta agcgcccccc ctctggtcct ccaccgcgtc
     6001 ttggaggaca cccagcccat cacccaaacg ggggacaacg cttatctggt cccgaggcag
     6061 ggcatccttc ttcagctcgc ccacacgctc accctcaacg gggccttctc caacgcgtgg
     6121 gatcgggctt tcgtccgcct taaccgcacc gataccatca tggaggttat ccccggcgtt
     6181 cattacgccc tggacctcca ggggcgcaac gctcaaacct ctcccatcac tccaactcac
     6241 gtcatcttcc acgacctctt ccgggctatg ggggcgctgg accgggggga cctccgggat
     6301 gccttggaca cggaggccgt tacaactacc gagagcgtgg taacggtcag ccaaagcgcc
     6361 accctggggg ccaacaacaa cttcttgggg gccattcggc gcatcattca accgattagg
     6421 gtttagtggg aggtaggaga tgctgagcga ttacgttgcc ctcaataacg ttctcccctc
     6481 cgttggaggg tggagcttgg gggagcaggc cacggcggga ctgcaggacg ctacgcaacc
     6541 cgccaccacc gctagcggga agacccccac ggggcagggg gccctcctgg aaggacaaag
     6601 ccccctggtt actttcctgg tggtccttgg cttcctgctc ctcctcatgt ggttggctca
     6661 cacctacggg gagccggggg aatactccaa cctcaaggcg agtgcctaca acgtgctgtt
     6721 catcgggctt acggcggctc tgttcatccc cctcctcaag gtggcggcgg tcaagattcc
     6781 cggcccgtgg actagctact tcctgagcat ttaggggggg gcatgtggga gtacctgcag
     6841 gctaaggccc aggagtggct ggggagaacc gggttgacct tccaggtcgt cctggtcctg
     6901 gtgctcctct acttgggcta ccgtgcaggt accccgtggc ttatcgcggc tatcctggtg
     6961 agcatggtgg tcttggcggg gggtggcggt gagtaggctc gggtgggcga ttatcgcggc
     7021 ggtggtgctc gccctcaccg ccacctactt ccgccctctc tggatagtcg tggggctagt
     7081 cattttgatt ttcctgctga acgcagtagg aggagggagg tagcatggac accgtggaaa
     7141 agattgcact cacggccttg gcgattgtag ccgtcattgt cgtcctgcag aggggcacgg
     7201 cggcggcgca agtggtgaac gccatcggga gcacgtacac caacttggta aagacgctgg
     7261 tgagcgcgcc caagtagggg gggagccatg tgcaatcgtg aggtggccct tccccgtagc
     7321 cgctacgccc aggctctccg ggatgaggca gggcttccca cgaggcctta cgactactac
     7381 tcccgcccgt ccatgtaccc tggtcccgtg gggcccgtcc ccgccccccc cactatcccg
     7441 gtgcctaccc ctatcggcat cttgcctggg tacaggggag aacttagcgc cctcaagtat
     7501 cagggggtag tgcctaggga gcacccggca gggccctacg cgcccgaagg tgggtacatc
     7561 acatgggcgg atgtggtgaa gaggaggggc gatggaggtt aaggactacc ttccttttgc
     7621 cgttgtggga gggggggccc ttcttgccta cctgctcctc aagccccagc cctacacggt
     7681 agtcaatccc cctactcctc aagggcctag cgaggatgcg aggctcaacg ctgtcgtggc
     7741 tttgactcag ctcgcagtgg aaagggaaag ggcccggtgg gagtacgccg ccacggtgga
     7801 ggccgctaaa gcccaagcgg agtacgagct tagggcccgt cagattgagg ctgagcaaga
     7861 ggcgcggcga atccaggcag aaaaggaggt acagcagaaa gcggcagacg cgcagttttg
     7921 gggaacgttc tggtccggtc tttttggaat aatcgggagc gggctggccc ttttcgcggg
     7981 gctttccgat gaagccctct acaaccgcct tccttatccg ccctacatcg ggcacggcgg
     8041 gcaactcgcg gccctctccc ggaggaggtg agcatgggga ttgcgattga gcctattgac
     8101 tccggtccat gcgtggactc ggactgctac tatgcgcggg gggagagatt gacctggtgg
     8161 gaggcccctc ctccccctcc tccttccaat cccgcgcttg accccccccc tcctcctcct
     8221 cctcagcggg aggagcgccc tcggatagac cctcccccca cgctttttga ccagccctca
     8281 tactctgcag aacccccccc ctcccctcct ccttctacgc caagccctcc ggtggacccc
     8341 gagccccctc ccgacccctt gcctccagta acctcggacg acccgcttta ttcggtaatc
     8401 ggggaactcc tggggggcag ggagcctacc gcgcaaccag agcccatagt gctcgcccct
     8461 cccccctcta atccgtcttt ggctctttgg attatggccc ttgcggtggt gggagtgttg
     8521 gggtacgcgt ggcttcagag gaggtgaaca tggactgggg caaactcatt ggcttccggc
     8581 ggtaccagag gcagaaccct gatgcgtatg tggcccccac atcccccctc ccggagtggc
     8641 cccctcttcc ccagaccggg actttcgccc attgggggga tgacgttctg gccaatctct
     8701 ccggcggtgc ggctcgggcc atgtgggatt ggcccgcgag atacgccacg gtggaagcgt
     8761 tcaccccccc ggaggtagtc ctaaggtggc ctacgcctta gatgtctacc agcttctaag
     8821 ggactgcacg gggcgggacg acttggcccg ccttgggacg gccattttcc tccatgagaa
     8881 gggccctcag gggtatgagg cagggtatgg ctacacggat agcggccccc tcccggagtg
     8941 gcggggcttt cagaagcaaa tccaaggggt atgctctgaa cttacgcgct tcttccaggg
     9001 gaggcccgtc aacgcctaca cggtgcggga gttttggcga gaggaatggc gcgcaagcga
     9061 tatcgggtgg ccgcaaaagg tttgggcttg gtatttgcgt ttggcgggag cgcccaccga
     9121 caagctcgcc caaatgccta tcgggagagg ggaggcctcc cgcccctccc ctctccttcc
     9181 ccgtatcccc acggtggaag ataaactgca gggtagcctt tccccccgcg tacaagaggt
     9241 tttggaacag cgcacccctg aggcggtgag gaaggccctt ctcatcggcc tcggggccat
     9301 tggactagcc gtggtatggt atgaggtagg gaggagagta tgagtctgac accgcaagga
     9361 gtaagtggaa acagtgtcgg aggggatagc tcttccagcg cgtggccctc taccctggtc
     9421 gctcccggta ctatttacca ccttggacgc tggacggggc agaccctacg caagctgata
     9481 ctatccaccg ccaccgattc tattgtctat gggcgcgttc agatagcctt ggactaccca
     9541 cgggggggac tgcatccggg caacaacgcc cttcctaacg ccttggtaga cctcccccta
     9601 aactttagcc cccaacatcc catcataagc ttggacttca caccaggact agtaactgtc
     9661 ttgccggggg aaagcatcta cctcatcgtg tatgacttca tgagcacgac ttccgcggca
     9721 ataacttggg ggcttaacat gttttgggag cctttgggag gtgaaagatg agcctggaga
     9781 ttttcccaac ttatcccgaa atacgcacag aacagcttag cgccgtggag tgggataaac
     9841 ttcccaacct gtccacgctc gtaatcagcg tgatccgcaa cgcccctacc atcacgcctt
     9901 cccccggaga ctggctcctc ggggtgctgg ctggcggtac cgaggtaggc aagttgccca
     9961 tcggccaagc cgacggtgta cccctactca atgccagcgg tgccctcgcg agcgccgacg
    10021 cgtacctgag cagggggtct gtgaccagcg ctgtagactg gaacaccctc accaatgcgg
    10081 gtacatatga gatagcgagc ggggccttcg gcaccggtag cgccaacacg ccgcccgccg
    10141 tgacccagca ggggcacctg ctggttctca aaacatcggg ggccaccacc caggtgtacg
    10201 tccctaaaag cgccgaccct ggcatctatt ggcggcagta cgccgggggc acctggtctt
    10261 cctgggccac ggccggtgtg atgtatgggt ccaactctaa cggctcctac attcggtttg
    10321 cggatggaac gcagatatgc tggcgtgaag caacttggga aatgccaacg ggatggagta
    10381 caggtccctc tgtcccttgg cctgcggcgt ttgtggccac acccagggtg tggcttaact
    10441 atactagatc ggggtggcgc atcatcctca gcgatattac caacaatagt actaccatcc
    10501 aattgtccgc ctacaacttt gacaccacag taacgccgta cagggtggac atactagcca
    10561 tcgggaggtg gaagtgattc ggattcacta cgttccgcaa gttgggctcc cgggcaagcg
    10621 cctagtctac tcctggagcg gacgcgtgct cactgtgcgc ctggaacctg acggggtaga
    10681 agatgtctac gatttgagcg ttctaggccc gggtgatgag gtggtagggg ttgagacgga
    10741 gaccctgccc tttactccaa tcctttcggc tcgcttagag attgacgggg atttgcatgt
    10801 aacgctccta cattggtacg aagggggaga ggagcccgag ctggctgagg aggtactaga
    10861 tggctagact aaaaatcaaa acccaccagg accgcatccg ggaggcctgg gcggccctta
    10921 gggccgagcg tgaccgccgc ctggcggaga cggactgggt ggtggcccgg gcctatgagc
    10981 ggggggagcc cgtgccggag gcctgggcgg cctaccgcca ggccctccgg gacctcccgg
    11041 ctctagtggg cgaagaagac cttctggcag ggcgcatccc ctggcctgag ccacctgggg
    11101 gggcttcata aacactcccc ccaaccctg
//