Laboratory of Molecular Genetics of Microorganisms

All strains

Thermus sp. K25-4

Genus
Thermus
Species
sp.
Strain
K25-4
Cultivation medium
Thermus TB++ medium
Cultivation temperature, °C
70
Genome size, bp
2 516 994
GC content, %
69
Assembly level
Large contigs
Isolation source
Kunashir Island
Received
September 1, 2025

Sequences

FileKindVersionSequencingAssemblerSize
Tsp_K25-4_genome.gb.gzannotationMinION + DNBSEQ-G400, PE hybracter v0.10.01.6 MB

Replicons

Table (5)
IDNameTopologyLength, bpGC content, %
K25_4_CHRchromosomecircular2 017 67669.1
pK25_4_1plasmid pK25_4_1linear356 81768.9
pK25_4_2plasmid pK25_4_2circular114 32166.4
pK25_4_3plasmid pK25_4_3circular15 76967.6
pK25_4_4plasmid pK25_4_4circular12 41167.8

CRISPR-Cas systems

Table (3)
SubtypeRepliconCoordinates
I-Eplasmid pK25_4_180 400–89 928
III-Bplasmid pK25_4_1143 352–149 639
III-Aplasmid pK25_4_1156 388–168 748

I-Eplasmid pK25_4_1 · 80 400..89 928 · 9 529 bp · 9 coding sequences

CRISPR array — 77 801..77 953 · 153 bp · repeat consensus: CGGTCCATCCCCACGTGCGTGGGGACTACGMobile element — 77 992..79 135 · 1 144 bpCRISPR array — 79 140..80 349 · 1 210 bp · repeat consensus: CGGTCCATCCCCACGTGCGTGGGGACTACCRISPR-Cas I-E — 80 400..89 928 · 9 529 bpCRISPR array — 90 119..91 006 · 888 bp · repeat consensus: GTAGTCCCCACATGCGTGGGGATGGACCGTRAP transporter large permease protein — 75 801..76 343 (+) · 543 bp · partial frameZn-dependent hydrolase, glyoxylase — 76 346..77 032 (+) · 687 bp5-oxoprolinase subunit A — 77 045..77 797 (+) · 753 bpTransposase IS4-like domain-containing protein — 78 004..78 543 (−) · 540 bpH repeat-associated protein N-terminal domain-containing protein — 78 540..79 094 (−) · 555 bpCRISPR-associated endoribonuclease Cas2 — 80 400..80 777 (−) · 378 bpCRISPR-associated endonuclease Cas1 2 — 80 737..81 708 (−) · 972 bpCRISPR-associated endoribonuclease Cse3 — 81 718..82 353 (−) · 636 bpType I-E CRISPR-associated protein Cas5/CasD — 82 343..83 014 (−) · 672 bpType I-E CRISPR-associated protein Cas7/Cse4/CasC — 83 016..84 131 (−) · 1 116 bpCRISPR-associated protein Cse2 — 84 128..84 625 (−) · 498 bpCRISPR-associated protein CasA/Cse1 — 84 622..86 100 (−) · 1 479 bpCRISPR-associated endonuclease/helicase Cas3 — 86 159..88 942 (−) · 2 784 bpTranscriptional regulator — 88 939..89 928 (−) · 990 bpDNA methylase N-4/N-6 domain-containing protein — 91 099..92 055 (+) · 957 bphypothetical protein — 92 052..92 933 (+) · 882 bpVWFA domain-containing protein — 92 930..93 006 (+) · 77 bp · partial frame

Integrative elements

Table (3)
NametRNARepliconCoordinatesLength, bp
IE1tRNA-Ilechromosome267 034–301 46634 433
IE2tRNA-Argchromosome689 980–697 6547 675
IE3tRNA-Valchromosome1 092 794–1 100 1327 339

IE1chromosome · 267 034..301 466 · 34 433 bp · 29 coding sequences · 1 tRNA

Attachment site — 267 034..267 110 · 77 bpMobile element — 267 034..301 466 · 34 433 bpMobile element — 296 777..297 692 · 916 bpAttachment site — 301 420..301 466 · 47 bpDprA winged helix domain-containing protein — 265 034..265 070 (−) · 37 bp · partial frameGTPase Era — 265 155..266 060 (+) · 906 bpPutative acetyltransferase — 266 105..266 992 (+) · 888 bpSite-specific integrase — 267 187..268 362 (−) · 1 176 bpNucleoid occlusion protein — 268 517..269 542 (+) · 1 026 bphypothetical protein — 269 543..270 049 (+) · 507 bphypothetical protein — 270 245..270 832 (−) · 588 bpAAA+ ATPase domain-containing protein — 270 820..271 983 (−) · 1 164 bpNrS-1 polymerase-like HBD domain-containing protein — 271 974..272 993 (−) · 1 020 bphypothetical protein — 273 010..273 270 (−) · 261 bpputative excisionase — 273 693..274 007 (+) · 315 bpMicrococcal nuclease-like protein — 274 038..274 730 (+) · 693 bphypothetical protein — 274 727..275 404 (−) · 678 bphypothetical protein — 275 385..275 552 (−) · 168 bpSwt1-like HEPN domain-containing protein — 275 695..278 673 (−) · 2 979 bpSigma-70, region 4 — 278 730..279 281 (−) · 552 bpHelix-turn-helix domain protein — 279 286..279 711 (−) · 426 bpAddiction module toxin RelE — 279 714..280 076 (−) · 363 bpAdenine-specific DNA methylase — 280 119..282 953 (−) · 2 835 bpTransposase for transposon Tn5 — 284 234..285 361 (−) · 1 128 bpBeta-glucosidase A — 285 592..287 289 (+) · 1 698 bpMFS transporter — 287 663..288 196 (−) · 534 bpMFS transporter — 288 397..289 593 (−) · 1 197 bpRadical SAM core domain-containing protein — 289 586..291 061 (−) · 1 476 bpBacterial transcriptional activator domain-containing protein — 291 327..292 811 (−) · 1 485 bphypothetical protein — 292 909..293 148 (−) · 240 bpTransposase — 293 096..294 364 (−) · 1 269 bpTransposase IS4 family protein — 294 698..295 567 (−) · 870 bpIS256 family ISTth4 transposase — 295 589..296 821 (+) · 1 233 bpInsertion element IS402-like domain-containing protein — 296 811..297 218 (+) · 408 bpTransposase IS4-like domain-containing protein — 297 236..297 661 (+) · 426 bpDEAD/DEAH box helicase family protein — 297 825..301 214 (−) · 3 390 bpBifunctional oligoribonuclease and PAP phosphatase NrnA — 301 545..302 522 (+) · 978 bpPutative restriction endonuclease domain-containing protein — 302 500..303 048 (−) · 549 bpputative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase — 303 058..303 466 (−) · 409 bp · partial frametRNA-Ile(gat) — 267 034..267 110 (+) · 77 bp