Laboratory of Molecular Genetics of Microorganisms

All strains

Thermus sp. K25-3

Genus
Thermus
Species
sp.
Strain
K25-3
Cultivation medium
Thermus TB++ medium
Cultivation temperature, °C
70
Genome size, bp
2 405 087
GC content, %
69
Assembly level
Complete replicons
Isolation source
Kunashir Island
Received
September 1, 2025

Sequences

FileKindVersionSequencingAssemblerSize
Tsp_K25-3_genome.gb.gzannotationMinION + DNBSEQ-G400, PE hybracter v0.10.01.5 MB

Replicons

Table (3)
IDNameTopologyLength, bpGC content, %
K25_3_CHRchromosomecircular2 015 43569.1
pK25_3_1plasmid pK25_3_1circular373 43568.3
pK25_3_2plasmid pK25_3_2circular16 21766.4

CRISPR-Cas systems

Table (2)
SubtypeRepliconCoordinates
III-Bplasmid pK25_3_1169 794–176 078
I-Eplasmid pK25_3_1229 083–239 694

III-Bplasmid pK25_3_1 · 169 794..176 078 · 6 285 bp · 6 coding sequences

CRISPR array — 164 689..165 027 · 339 bp · repeat consensus: GTTGCAAACCACTTAGCCCCGTAAGGGGATTGCGACCRISPR-Cas III-B — 169 794..176 078 · 6 285 bpCsm6 6H domain-containing protein — 162 689..163 385 (+) · 697 bp · partial frameCRISPR-associated endonuclease Cas1 1 — 163 389..164 336 (+) · 948 bpCRISPR-associated protein — 165 208..167 112 (+) · 1 905 bpDUF4145 domain-containing protein — 167 089..167 610 (−) · 522 bpSirohydrochlorin cobaltochelatase — 167 615..168 334 (−) · 720 bpDUF2207 domain-containing protein — 168 303..169 268 (−) · 966 bphypothetical protein — 169 261..169 668 (−) · 408 bpGGDEF domain-containing protein — 169 794..171 548 (+) · 1 755 bpCRISPR-associated protein Cmr3 — 171 538..172 620 (+) · 1 083 bpType III-B CRISPR module RAMP protein Cmr1 — 172 617..173 807 (+) · 1 191 bpType III-B CRISPR module RAMP protein Cmr4 — 173 819..174 679 (+) · 861 bpCRISPR system Cmr subunit Cmr5 — 174 676..175 026 (+) · 351 bpRAMP superfamily protein — 175 029..176 078 (+) · 1 050 bpLipoprotein — 176 204..177 388 (+) · 1 185 bphypothetical protein — 177 385..177 798 (+) · 414 bpManganese-binding lipoprotein MntA — 177 795..178 078 (+) · 284 bp · partial frame

Integrative elements

Table (2)
NametRNARepliconCoordinatesLength, bp
IE1tRNA-Argchromosome678 533–686 2047 672
IE2tRNA-Thrchromosome1 813 122–1 822 1639 042

IE1chromosome · 678 533..686 204 · 7 672 bp · 7 coding sequences · 2 tRNAs

Attachment site — 678 533..678 578 · 46 bpMobile element — 678 533..686 204 · 7 672 bpAttachment site — 686 129..686 204 · 76 bpPhosphoenolpyruvate carboxylase — 676 533..678 500 (+) · 1 968 bp · partial framehypothetical protein — 678 831..679 277 (+) · 447 bpHelix-turn-helix domain-containing protein — 679 356..679 565 (+) · 210 bpProtein YfjI — 679 725..682 010 (+) · 2 286 bpDNA polymerase I — 682 330..684 003 (+) · 1 674 bphypothetical protein — 684 111..684 365 (+) · 255 bphypothetical protein — 684 362..684 583 (+) · 222 bpTransposase — 684 849..685 991 (+) · 1 143 bpSec-independent protein translocase protein TatA — 686 465..686 626 (+) · 162 bpO-linked GlcNAc transferase — 686 623..687 690 (−) · 1 068 bpATP-dependent protease ATPase subunit HslU — 687 710..688 204 (−) · 495 bp · partial frametRNA-Ser(cga) — 678 533..678 608 (−) · 76 bp · pseudogenetRNA-Arg(tct) — 686 129..686 204 (−) · 76 bptRNA-Gly(tcc) — 686 240..686 316 (−) · 77 bptRNA-Pro(tgg) — 686 318..686 394 (−) · 77 bp