Thermus oshimai VZ2

Genus
Thermus
Species
oshimai
Strain
VZ2
Cultivation medium
Castenholz medium
Cultivation temperature, °C
70
Genome size, bp
2 222 563
GC content, %
68.8
Assembly level
Large contigs
Isolation source
Golyginskiye Klyuchi, Kamchatka

Sequences

FileKindVersionSequencingAssemblerSize
TOSH_VZ2_genome.gb.gzannotationMinION hybracter v0.10.01.4 MB

Replicons

Table (5)
IDNameTopologyLength, bpGC content, %
VZ2_CHRchromosomecircular1 969 86468.8
pVZ2_1plasmid pVZ2_1circular237 16468.7
pVZ2_2plasmid pVZ2_2linear10 18969.0
pVZ2_3plasmid pVZ2_3linear3 72971.4
pVZ2_4plasmid pVZ2_4linear1 61770.1

CRISPR-Cas systems

Table (2)
SubtypeRepliconCoordinates
I-Echromosome1 103 528–1 113 064
III-Achromosome1 208 612–1 222 230

I-Echromosome · 1 103 528..1 113 064 · 9 537 bp · 9 coding sequences

CRISPR array — 1 099 910..1 103 470 · 3 561 bp · repeat consensus: CGGTCCATCCCCACGCGTGTGGGGACTACCRISPR array — 1 113 228..1 114 120 · 893 bp · repeat consensus: GTAGTCCCCACGCCCGTGGGGATGGACCG16S rRNA (Guanine(966)-N(2))-methyltransferase RsmD — 1 097 910..1 098 255 (−) · 346 bp · partial frameNa+/phosphate symporter — 1 098 266..1 099 744 (−) · 1 479 bpCRISPR-associated endoribonuclease Cas2 — 1 103 528..1 103 884 (−) · 357 bpCRISPR-associated endonuclease Cas1 2 — 1 103 844..1 104 815 (−) · 972 bpCRISPR-associated endoribonuclease Cse3 — 1 104 819..1 105 436 (−) · 618 bpType I-E CRISPR-associated protein Cas5/CasD — 1 105 411..1 106 166 (−) · 756 bpType I-E CRISPR-associated protein Cas7/Cse4/CasC — 1 106 168..1 107 298 (−) · 1 131 bpCRISPR-associated protein Cse2 — 1 107 295..1 107 795 (−) · 501 bpCRISPR-associated protein CasA/Cse1 — 1 107 792..1 109 270 (−) · 1 479 bpCRISPR-associated endonuclease/helicase Cas3 — 1 109 328..1 112 078 (−) · 2 751 bpTranscriptional regulator — 1 112 093..1 113 064 (−) · 972 bpGlycerol-3-phosphate acyltransferase — 1 114 129..1 114 635 (+) · 507 bpDephospho-CoA kinase — 1 114 577..1 115 203 (−) · 627 bpCC-adding tRNA nucleotidyltransferase — 1 115 237..1 116 120 (+) · 884 bp · partial frametRNA-Pro(cgg) — 1 099 789..1 099 865 (−) · 77 bp

Integrative elements

Table (1)
NametRNARepliconCoordinatesLength, bp
IE1tRNA-Argchromosome915 690–923 6527 963

IE1chromosome · 915 690..923 652 · 7 963 bp · 6 coding sequences · 2 tRNAs

Attachment site — 915 690..915 735 · 46 bpMobile element — 915 690..923 652 · 7 963 bpAttachment site — 923 577..923 652 · 76 bpRibosomal RNA small subunit methyltransferase H — 913 690..914 455 (−) · 766 bp · partial frameTranscriptional regulator MraZ — 914 459..914 893 (−) · 435 bphypothetical protein — 916 037..916 822 (+) · 786 bphypothetical protein — 916 878..917 114 (+) · 237 bpDNA primase/polymerase bifunctional N-terminal domain-containing protein — 917 322..919 679 (+) · 2 358 bpDNA polymerase I — 919 669..921 753 (+) · 2 085 bpTransposase — 921 882..922 319 (+) · 438 bpIntegrase — 922 347..923 501 (+) · 1 155 bpSec-independent protein translocase protein TatA — 923 909..924 070 (+) · 162 bpSulfur carrier protein FdhD — 924 067..924 804 (−) · 738 bpO-linked GlcNAc transferase — 924 902..925 652 (−) · 751 bp · partial frametRNA-Ser(cga) — 915 690..915 813 (−) · 124 bp · pseudogenetRNA-Arg(tct) — 923 577..923 652 (−) · 76 bptRNA-Gly(tcc) — 923 687..923 762 (−) · 76 bptRNA-Pro(tgg) — 923 764..923 840 (−) · 77 bp