Laboratory of Molecular Genetics of Microorganisms

All strains

Thermus hydrothermalis K25-5

Genus
Thermus
Species
hydrothermalis
Strain
K25-5
Cultivation medium
Thermus TB++ medium
Cultivation temperature, °C
70
Genome size, bp
2 426 330
GC content, %
67
Assembly level
Complete replicons
Isolation source
Kunashir Island
Received
September 1, 2025

Sequences

FileKindVersionSequencingAssemblerSize
THY_K25-5_genome.gb.gzannotationMinION + DNBSEQ-G400, PE hybracter v0.10.01.6 MB

Replicons

Table (2)
IDNameTopologyLength, bpGC content, %
K25_5_CHRchromosomecircular2 111 07167.1
pK25_5_1plasmid pK25_5_1circular315 25966.2

CRISPR-Cas systems

Table (1)
SubtypeRepliconCoordinates
I-Eplasmid pK25_5_1161 217–170 606

I-Eplasmid pK25_5_1 · 161 217..170 606 · 9 390 bp · 8 coding sequences

CRISPR array — 160 568..161 158 · 591 bp · repeat consensus: GGTCCATCCCCACGCGTGTGGGGACTACCRISPR-Cas I-E — 161 217..170 606 · 9 390 bpCRISPR array — 170 849..172 103 · 1 255 bp · repeat consensus: GTAGTCCCCACGCACGTGGGGATGGACChypothetical protein — 158 569..158 647 (−) · 79 bp · partial framePD-(D/E)XK endonuclease-like domain-containing protein — 158 814..159 548 (−) · 735 bphypothetical protein — 160 221..160 355 (−) · 135 bphypothetical protein — 160 368..160 535 (−) · 168 bpCRISPR-associated endoribonuclease Cas2 — 161 217..161 561 (−) · 345 bpCRISPR-associated endonuclease Cas1 2 — 161 521..162 489 (−) · 969 bpCRISPR-associated endoribonuclease Cse3 — 162 498..163 130 (−) · 633 bpType I-E CRISPR-associated protein Cas5/CasD — 163 120..163 791 (−) · 672 bpType I-E CRISPR-associated protein Cas7/Cse4/CasC — 163 793..164 911 (−) · 1 119 bpCRISPR-associated protein Cse2 — 164 908..165 408 (−) · 501 bpCRISPR-associated protein CasA/Cse1 — 165 405..166 805 (−) · 1 401 bpCRISPR-associated endonuclease/helicase Cas3 — 166 898..169 663 (−) · 2 766 bpTranscriptional regulator — 169 674..170 642 (−) · 969 bphypothetical protein — 173 637..173 873 (+) · 237 bphypothetical protein — 173 863..174 103 (+) · 241 bp · partial frame

Integrative elements

Table (4)
NametRNARepliconCoordinatesLength, bp
IE1tRNA-Ilechromosome99 296–133 69434 399
IE2tRNA-Alachromosome334 753–365 46030 708
IE3tRNA-Alachromosome676 174–688 57812 405
IE4tRNA-Thrchromosome1 664 598–1 671 5146 917

IE1chromosome · 99 296..133 694 · 34 399 bp · 28 coding sequences · 1 tRNA

Attachment site — 99 296..99 342 · 47 bpMobile element — 99 296..133 694 · 34 399 bpMobile element — 103 070..103 976 · 907 bpAttachment site — 133 618..133 694 · 77 bphypothetical protein — 97 296..98 220 (+) · 925 bp · partial framehypothetical protein — 98 196..98 486 (+) · 291 bpTransglycosylase SLT domain protein — 98 464..99 180 (+) · 717 bpDEAD/DEAH box helicase family protein — 99 548..102 937 (+) · 3 390 bpTransposase IS4-like domain-containing protein — 103 101..103 502 (−) · 402 bpInsertion element IS402-like domain-containing protein — 103 535..103 942 (−) · 408 bpIS256 family ISTth4 transposase — 103 932..105 140 (−) · 1 209 bpTransposase IS4 family protein — 105 162..106 031 (+) · 870 bpTransposase — 106 365..107 633 (+) · 1 269 bphypothetical protein — 107 581..107 820 (+) · 240 bpBacterial transcriptional activator domain-containing protein — 107 918..109 402 (+) · 1 485 bpRadical SAM core domain-containing protein — 109 668..111 143 (+) · 1 476 bpMFS transporter — 111 136..112 332 (+) · 1 197 bpMFS transporter — 112 533..113 066 (+) · 534 bpBeta-glucosidase A — 113 440..115 137 (−) · 1 698 bpTransposase for insertion sequence element IS905 — 116 115..117 698 (−) · 1 584 bpAdenine-specific DNA methylase — 117 776..120 610 (+) · 2 835 bpAddiction module toxin RelE — 120 653..121 015 (+) · 363 bpHelix-turn-helix domain protein — 121 018..121 443 (+) · 426 bpSigma-70, region 4 — 121 448..121 999 (+) · 552 bpSwt1-like HEPN domain-containing protein — 122 056..125 343 (+) · 3 288 bphypothetical protein — 125 324..126 001 (+) · 678 bpMicrococcal nuclease-like protein — 125 998..126 690 (−) · 693 bpputative excisionase — 126 721..127 035 (−) · 315 bphypothetical protein — 127 458..127 718 (+) · 261 bpNrS-1 polymerase-like HBD domain-containing protein — 127 735..128 754 (+) · 1 020 bpAAA+ ATPase domain-containing protein — 128 745..129 908 (+) · 1 164 bphypothetical protein — 129 896..130 483 (+) · 588 bphypothetical protein — 130 679..131 185 (−) · 507 bpNucleoid occlusion protein — 131 186..132 211 (−) · 1 026 bpSite-specific integrase — 132 366..133 541 (+) · 1 176 bpCorrinoid adenosyltransferase — 134 010..134 582 (+) · 573 bpTransposase — 134 579..134 863 (−) · 285 bpSulfate-transporting ATPase — 134 918..135 637 (+) · 720 bpSodium ABC transporter, permease protein NatB — 135 634..135 694 (+) · 61 bp · partial frametRNA-Ile(gat) — 133 618..133 694 (−) · 77 bp