Laboratory of Molecular Genetics of Microorganisms

All bacteriophages

Thermus phage phiFa

Host
Thermus thermophilus HB27c
Morphotype
siphovirus
Lifecycle
lytic
Genome type
dsDNA
Genome length, bp
77 664
GC content, %
59.2
Accession
MH673672

Genome map

phiFa77 664 bp · 91 coding sequences · 1 tRNA

Direct terminal repeat — 1..6 638 · 6 638 bpDirect terminal repeat — 71 022..77 659 · 6 638 bphypothetical protein — 424..885 (+) · 462 bphypothetical protein — 986..1 171 (+) · 186 bphypothetical protein — 1 264..1 518 (+) · 255 bphypothetical protein — 1 629..1 802 (+) · 174 bphypothetical protein — 2 133..2 369 (+) · 237 bpRNA polymerase — 2 564..4 669 (+) · 2 106 bphypothetical protein — 5 219..5 458 (+) · 240 bphypothetical protein — 5 489..5 872 (+) · 384 bphypothetical protein — 6 073..6 348 (+) · 276 bphypothetical protein — 6 405..6 599 (+) · 195 bphypothetical protein — 7 131..7 427 (+) · 297 bphypothetical protein — 7 495..7 773 (+) · 279 bphypothetical protein — 7 781..8 485 (+) · 705 bphypothetical protein — 8 498..9 280 (+) · 783 bpAAA+ superfamily ATPase — 9 302..10 309 (+) · 1 008 bphypothetical protein — 10 370..10 588 (+) · 219 bpribonuclease H — 10 600..11 097 (+) · 498 bphypothetical protein — 11 105..11 332 (+) · 228 bphypothetical protein — 11 322..12 470 (+) · 1 149 bphypothetical protein — 12 615..13 148 (+) · 534 bphypothetical protein — 13 136..13 651 (+) · 516 bphypothetical protein — 13 651..14 394 (+) · 744 bphypothetical protein — 14 391..15 479 (+) · 1 089 bphypothetical protein — 15 490..16 332 (+) · 843 bphypothetical protein — 16 329..16 814 (+) · 486 bphypothetical protein — 16 845..17 669 (+) · 825 bphypothetical protein — 17 662..18 177 (+) · 516 bprecombination DNA repair protein — 18 208..18 957 (+) · 750 bpreplicative DNA helicase — 19 029..20 189 (+) · 1 161 bpRecB family exonuclease — 20 143..20 976 (+) · 834 bphypothetical protein — 20 978..21 460 (+) · 483 bpholliday junction resolvase — 21 460..21 894 (+) · 435 bpDNA primase small subunit — 22 222..23 235 (+) · 1 014 bphypothetical protein — 23 193..23 741 (+) · 549 bpXerD-like integrase — 23 803..24 660 (−) · 858 bphypothetical protein — 24 979..25 503 (+) · 525 bpdeoxycytidine triphosphate deaminase — 25 439..25 972 (+) · 534 bphypothetical protein — 25 975..26 289 (+) · 315 bphypothetical protein — 26 361..27 014 (+) · 654 bpdNMP kinase — 27 017..27 532 (+) · 516 bphypothetical protein — 27 532..27 807 (+) · 276 bpXRE family transcriptional regulator — 27 692..28 294 (+) · 603 bpFAD-dependent thymidylate synthase — 28 353..29 114 (+) · 762 bpribonucleoside-triphosphate reductase — 29 111..31 078 (+) · 1 968 bphypothetical protein — 31 118..31 294 (+) · 177 bphypothetical protein — 31 281..31 916 (+) · 636 bpDNA polymerase I — 31 846..34 092 (+) · 2 247 bphypothetical protein — 34 079..34 576 (+) · 498 bphypothetical protein — 34 628..34 828 (−) · 201 bphypothetical protein — 34 831..35 121 (−) · 291 bphypothetical protein — 35 118..35 486 (−) · 369 bpmuramidase — 35 486..36 025 (−) · 540 bphypothetical protein — 36 009..36 209 (−) · 201 bphypothetical protein — 36 187..36 555 (−) · 369 bphypothetical protein — 36 634..36 879 (−) · 246 bphypothetical protein — 36 891..39 416 (−) · 2 526 bphypothetical protein — 39 425..40 531 (−) · 1 107 bphypothetical protein — 40 541..42 376 (−) · 1 836 bpholin — 42 379..42 846 (−) · 468 bphypothetical protein — 42 839..43 612 (−) · 774 bphypothetical protein — 43 665..44 201 (−) · 537 bptail fiber protein — 44 214..45 125 (−) · 912 bpdistal tail fiber protein — 45 129..50 195 (−) · 5 067 bptape tail measure protein — 50 244..58 553 (−) · 8 310 bphypothetical protein — 58 555..58 737 (−) · 183 bphypothetical protein — 58 764..59 129 (−) · 366 bpmajor capsid protein — 59 191..60 213 (−) · 1 023 bphypothetical protein — 60 213..60 404 (−) · 192 bpcapsid protein — 60 395..60 883 (−) · 489 bphypothetical protein — 60 886..61 713 (−) · 828 bpcapsid protein — 61 710..62 120 (−) · 411 bphypothetical protein — 62 123..62 326 (−) · 204 bpmajor head protein — 62 399..63 568 (−) · 1 170 bpcapsid protein — 63 577..64 188 (−) · 612 bpcapsid scaffolding protein — 64 199..65 173 (−) · 975 bpportal protein — 65 170..66 573 (−) · 1 404 bpterminase large subunit — 66 582..68 000 (−) · 1 419 bpterminase small subunit — 68 169..68 705 (−) · 537 bpParB-like nuclease/partitioning protein — 68 702..69 418 (−) · 717 bphypothetical protein — 69 418..70 101 (−) · 684 bphypothetical protein — 70 202..70 963 (−) · 762 bphypothetical protein — 71 456..71 917 (+) · 462 bphypothetical protein — 72 018..72 203 (+) · 186 bphypothetical protein — 72 296..72 550 (+) · 255 bphypothetical protein — 72 661..72 834 (+) · 174 bphypothetical protein — 73 165..73 401 (+) · 237 bpRNA polymerase — 73 596..75 701 (+) · 2 106 bphypothetical protein — 76 251..76 490 (+) · 240 bphypothetical protein — 76 521..76 904 (+) · 384 bphypothetical protein — 77 105..77 380 (+) · 276 bphypothetical protein — 77 437..77 631 (+) · 195 bptRNA-Lys — 24 743..24 810 (−) · 68 bp

Sequences

FileKindVersionSize
phiFa_genome.gb.gzannotation52 KB

Description

A siphovirus with a very long (~600 nm) tail. The genome contains two 6,638-bp direct terminal repeats (DTRs). The repeats encode early-expressed genes, including a phage double-ψ β-barrel RNA polymerase (phiFa_44). The DTRs also encode a set of small proteins that may function as anti-defense or host-takeover proteins.

The phage also produces plaques on the T. thermophilus HB8 lawn, albeit the PFU numbers are decreased by a few orders of magnitude and the plaques are extremely small.


Related publications

  1. Spacer acquisition by Type III CRISPR–Cas system during bacteriophage infection of Thermus thermophilus

    Daria Artamonova et al.

    Nucleic Acids Research2020

  2. Natural diversity of CRISPR spacers of Thermus : evidence of local spacer acquisition and global spacer exchange

    Anna Lopatina et al.

    Philosophical Transactions of the Royal Society B: Biological Sciences2019